Effect of Chlorogenic Acid on the Secretion of Inflammatory Cytokines in Human Hepatic Stellate Cells Induced by TGF-β1
WANG Ye-qiu1, ZHANG Li-hong1, CHEN Qiao-yun1*, LI Jian-min2
Author information+
1. Jiamusi College, Heilongjiang University of Chinese Medicine, Jiamusi 154007, China; 2.First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Haerbin 150040, China
OBJECTIVE To observe the effect of chlorogenic acid on the secretion of inflammatory cytokines and regulation mechanism of P38MAPK, NF-κB signaling pathway in human hepatic stellate cells induced by TGF-β1. METHODS Different concentrations of CGA worked in normal and activated hepatic stellate cells to make sure the appropriate drug concentration.The exponential growth phase cells were randomly divided into normal HSC group, normal HSC+CGA group, after cultured 48 h, the cells were cultured with 0、50、100 mg·L-1 CGA for 24 h; HSC(TGF-β1) group、 HSC(TGF-β1 + CGA) group: after 24 h, the cells were induced by 10 μg·L-1 TGF-β1 for 24 h, and then cultured with 0, 50, 100 mg·L-1 CGA for 24 h. The expression of α-SMA protein was detected by immunocytochemistry, the expression of p-P38, P65 protein was detected by Western-blot, the expression of TNF-α, IL-6 mRNA was detected by real time quantitative PCR, and the content of TNF-α, IL-6 in the supernatant was detected by ELISA method. RESULTS The appropriate concentrations of CGA were 50 and 100 mg·L-1, these concentration has no effect on normal HSC(P>0.05); after stimulation by TGF-β1, the expression of α-SMA, p-P38, P65, TNF-α, IL-6 was increased(P<0.01), when activated HSC cells were treated with 50 and 100 mg·L-1 CGA, the expression of α-SMA, p-P38, P65, TNF-α, IL-6 was decreased(P<0.05, P<0.01). CONCLUSION CGA can inhibit the proliferation of activated HSC, regulate the secretion of inflammatory factors such as TNF-α, IL-6 by P38MAPK and NF-κB signaling pathway, inhibit the occurrence of liver fibrosis.
WANG Ye-qiu, ZHANG Li-hong, CHEN Qiao-yun, LI Jian-min.
Effect of Chlorogenic Acid on the Secretion of Inflammatory Cytokines in Human Hepatic Stellate Cells Induced by TGF-β1[J]. Chinese Pharmaceutical Journal, 2017, 52(6): 452-456 https://doi.org/10.11669/cpj.2017.06.007
[1] GUO R, YAN M. The research progress of cellular and molecular mechanism in liver fibrosis[J]. Chin J Liver Dis(Elect Vers)(中国肝脏病杂志:电子版),2012,4(4):57-62. [2] LU L S, LUO C, CHEN Z X. The research progress of liver fibrosis mechanism[J]. Guide China Med(中国医药指南), 2012,10(3):49-50. [3] SHI H T,SHI A M, SHANG B X, et al. Effect of chlorogenic acid on hepatic stellate cell proliferation,generation and degradation of extracelluar matrix[J]. J Sichuan Univ(Med Sci Ed)(四川大学学报:医学版), 2015,46(3):372-375. [4] WEI M, YANG X M, LIU J H, et al. The research progress of chlorogenic acid pharmacological effects[J]. Shaanxi J Tradit Chin Med(陕西中医), 2016, 37(4):511-512. [5] YANG H X, BAI Y F. Effect of chlorogenic acid on lipid peroxidation in rat with hepatic fibrosis[J]. China Pharm(中国药业), 2013, 22(23):3-4. [6] HUANG W W, CHEN Y M, GONG X G, et al. Protective effect of chlorogenic acid on fibrosis of liver in the rat with obstructive jaundice[J]. Global Tradit Chin Med(环球中医药), 2012, 5(8):561-565. [7] SONG K J, LIAO C X, LIU Y P, et al. Effect of activation and apoptosis of mogroside on hepatic stellate cell[J]. Chin Tradit Pat Med(中成药), 2014, 36(3):481-484. [8] BI M, JU J, WU F R, et al. Therapeutic effects and mechanisms of total flavonoids of Chrysanthemun indicum on liver fibrosis in rats[J]. Chin Pharm J(中国药学杂志), 2014, 49(5):367-370. [9] WHITEMAN M, CHEUNG N S, ZHU Y Z, et al. Hydrogesul-phide: a novel inhibitor of hypochlorous acidmediated oxidative damage in the brain[J]. Biochem Biophys Res Commun, 2005, 326(4):794-798. [10] SZUSTER-CIESIELSKA A, PLEWKA K, DANILUK J, et al. Betulin and betulinic acid attenuate ethanol-induced liver stellate cell activation by inhibiting reactive oxygen species (ROS), cytokine (TNF-α, TGF-β) production and by influencing intracellular signaling[J]. Toxicology, 2011, 280(3): 152-163.
PDF(1401 KB)
